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- W2388872678 abstract "Objective To investigate the effects and regulation mechanism of HCV core/ NS3/NS5A on expression of IFN-β in HepG2 cells. Methods HCV core/NS3/NS5A proteinswere cloned (pcDNA3.1/myc-His-core/NS3/NS5A) and expressed in HepG2 cells, then the expression level of IFN-β was detected by qRT-PCR, Western blot hybridization and ELISA. IFN-β promoter reporter vector (PGL4.10-IFN-β-P) was constructed and used for regulation mechanism study by luciferase assay. Results HCV core/NS3/NS5A proteins were successfully expressed in HepG2 cells post-transfection. Both the mRNA level and protein expression of IFN-β were significantly decreased (P 0.05) in the presence of NS3/NS5A protein. Luciferase assay revealed that NS3 /NS5A proteins downregulated IFN-β promoter activity (P 0.05). Meanwhile, HCV core protein had no significant effect on IFN-β expression. Conclusions Overexpression of NS3/NS5A could inhibit the expression of IFN-β by downregulating IFN-β promoter activity." @default.
- W2388872678 created "2016-06-24" @default.
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- W2388872678 date "2013-01-01" @default.
- W2388872678 modified "2023-09-27" @default.
- W2388872678 title "Effects and regulation mechanism of HCV core/NS3/NS5A on expression of IFN-β in HepG2 cells" @default.
- W2388872678 hasPublicationYear "2013" @default.
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