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- W2388890831 abstract "Objective To investigate the effect of hypertonic-hyperoncotic solution (HHS) on CD11b expression on the surface of human neutrophils which are stimulated by lipopolysaccharide (LPS) in vitro. Methods Neutrophils were isolated from fresh peripheral venous blood of healthy volunteers aged 20-4oyr find incubated with LPS 100 ng/ml al 37℃ in 5% CO2 incubator for 15, 30, 60min, 4, 12 or 24h. HHS was prepared with 10%NaCl and 10% hydroxyethyl starch. Neutrophils were incubated with 0.25% or 0.5%HHS for 10min, 1h or 4h. Some of the HHS treated neutrophils were further subjected to LPS stimulation for 30 mm. CDllb on the surface of neutrophils were measured by using immunofluorescence and flow cytometry. Results CD11b expression on neutrophils increased significantly after being exposed to LPS for 15min (P0.05) and reached its peak level at 30min and then remained unchanged. HHS prevented the up-regulation of CDllb expression induced by LPS. The inhibition was irrelated to the concentration of and time of exposure to HHS. Conclusion The up-regulation of CDllb expression on neutrophil induced by LPS stimulation is rapid. It can reach its peak level in a short time. HHS does not alter CD11b expression but prevents the LPS-induced up-regulation." @default.
- W2388890831 created "2016-06-24" @default.
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- W2388890831 date "2002-01-01" @default.
- W2388890831 modified "2023-09-25" @default.
- W2388890831 title "The effect of hypertonic-hyperoncotic solution on CD11b expression on the lipopolysaccharide-stimulated human neutrophils in vitro" @default.
- W2388890831 hasPublicationYear "2002" @default.
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