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• W2388892283 abstract "Environmental condition is a key factor affecting the outbreak of cucumber downy mildew. To develop effective bionomic control strategies, it is important to decipher the essential condition and restrictive factors influencing the occurrence of this disease. “High|temperature sealing greenhouse” , which was used to control cucumber downy mildews, is a representative of the ecological control technique of vegetable disease. This method has been developed for over 20 years, but it was limited because the detailed studies on the correlated environmental factors has been not yet carried out. This study were to understand the effects of high temperature regulation on the infection of Pseudoperonospora cubensis by using the method of combination of temperature and humidity, and find the optimum temperature and humidity to control the cucumber downy mildew. Thus this information will probably provide new theoretical foundation for ecological control.; Diseased leaves of cucumber downy mildew with mould produced by placing in moist chamber were incubated in a growth chamber set to 50, 45, 40, 35℃and relative humidity (RH) 50%, 60%, 70%, 80%, 90 %, respectively, for 2 hours. Plants were inoculated by placing droplets of sporangial suspension on the surfaces of cotyledon. The disease index was investigated 4 days after inoculation. The results indicated that at the same humidity, the pathogenicity of the pathogen was decreased with the temperature increasing when Pseudoperonospora cubensis was treated in a growth chamber at high temperature from 35 to 50℃ and RH from 50% to 90 % for 2 hours. At the same temperature, the pathogenicity was significantly reduced with the humidity increasing when the temperature ranged 40 to 50℃, which showed the above 40℃ high temperature and high humidity apparently has significant lethal action to Pseudoperonospora cubensis . Whereas the pathogenicity of the pathogen was not obviously reduced when treatment temperature was below 40℃.; And Pseudoperonospora cubensis with pathogenicity were treated in a growth chamber at 50, 45, 40 and 35℃and RH 60%, 80% for 30, 60, 90 and 120 minutes, respectively, then sporangial suspension(4×10+4 sporangia/ml) was prepared by washing sporangia from the treated leaves. The disease index was investigated 4 days after inoculation. The results showed that the pathogenicity of the pathogen was reduced with lengthening the treatment time when the RH was adjusted to 80% and the temperature ranged from 40℃ to 50℃. The pathogenicity became weak when the pathogen was treated for over 30 minutes at the temperature of 50℃ or 45℃, RH 80%, respectively. Remarkably, it was observed that the pathogenicity was significantly decreased when the pathogen was pathogenicity after treated for over 120 minutes at 40℃, RH 80%. ; These results showed that the effect of killing pathogen became more obvious with lengthening the time of the treatment when the temperature and RH was set to above 45℃ and 60 % respectively.; By the above result and the experiment which controlled cucumber downy mildew by the high temperature and high relative humidity , The research results indicated the seedling treated by 50℃ was seriously harmed , even killed by burning, the treatment of 45℃ for 2 hours also had slight harm to seedling. But the seedlings were not damaged when they were treated by 45℃ for 1 hour or 40℃ for 2 hours .These showed the ability of tolerance to heat of the host had limit. However, further field experiment still need to perform.;Therefore, at the same temperature, the control effects were different as the different periods when the treatment of high temperature and high relative humidity were carried out after inoculation, the control effect was more efficient, the time of infection was more short. The effect was best when the treatment was carried out 2 hours after inoculation. At that time, Pseudoperonospora cubensis on the leaves was treated by high temperature and high relative humidity; However, the effects of treatment that were" @default.
• W2388892283 created "2016-06-24" @default.
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• W2388892283 date "2001-01-01" @default.
• W2388892283 modified "2023-09-23" @default.
• W2388892283 title "The effect of high temperature regulation on the infection of Pseudoperonospora cubensis" @default.
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