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- W2389005718 abstract "To explore the immune response of B cell epitope based DNA vaccine, the P127-138 B epitope gene of DHBV Pre-S was cloned into the pECK plasmid constructed in our laboratory. This recombinant plasmid (pECK-b1b2) was transfected into the C2C12 muscle cell line and expression of specific B cell epitope was detected by DOT-EIA. Moreover, the BALB/c (H-2d) and C57BL/6 (H-2b, B6) were immunized by pECK-b1b2 plasmid, respectively. The specific antibody in serum in mice was determined with ELISA. The results showed that B cell epitope of DHBV Pre-S was expressed in the C2C12 cell transfected by pECK-b1b2. Specific antibody against B epitope in sera was also produced after DNA immunization and specific antibody in serum of C57BL/6 was higher than that in BALB/c. These results demonstrated that DNA vaccine based on B epitope could efficiently induce specific immune response with MHC restriction." @default.
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- W2389005718 date "2005-01-01" @default.
- W2389005718 modified "2023-09-22" @default.
- W2389005718 title "Characterization of immune response of DNA vaccine encoding B cell epitope" @default.
- W2389005718 hasPublicationYear "2005" @default.
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