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- W2389018081 abstract "The random amplified polymorphic DNA (RAPD) was used to study genetic diversity (among) four serotype Riemerella anatipestifer strains, five enteropathogenic Escherichia coli strains and five Salmonella anatum strains from ducks. 8 primers were selected from twenty 10 bp random primers. Totally 102 RAPD bands were identified, 1 of which was common all the accessions and 101 of which were polymorphic between any two accessions. The polymorphic bands accounted for 99.0% of the total amplified bands. 51 bands were from 4 strains of R.anatipestifer , 12 of which were common, and polymorphic rate was 76.5%. 46 bands were from 5 strains of S.anatum , 13 of which were common, and polymorphic rate was 71.7%. 48 bands were from 4 strains of E.coli, 4 of which were common, and polymorphic rate was 91.7%. The 535 bp band, the 1 227 bp band and the 330 bp band, which were amplified with the primer G12 selected from the above-mentioned eight primers, were characteristic for R.anatipestifer, E.coli and S.anatum respectively. The results showed that the primer G12 can be used as molecular marker to differentiate R.anatipestifer from E.coli or S.anatum and to differentiate E.coli from S.anatum." @default.
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- W2389018081 date "2004-01-01" @default.
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- W2389018081 title "Study of riemerella anatipestifer,enteropathogenic escherichia coli and saslmonella anatum from ducks by random amplified polymorphic DNA" @default.
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