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- W2389058082 abstract "The rncgene of E.colican encode the RNaseIII protein which can degrade most of dsRNA transcripts in E.coli.Thernc gene of origami(DE3) strain usingλ-dependent Red-mediated recombination systemand gene splicing by overlap extension PCRand the M-origamistrain deficient for RNaseIII.wat And achieved the vector of LMP480 that can express TMV MP dsRNA transcripts was amstueted and transformed it intoM-origamistrain by electroporation.Induced by IPTG,M-origami/LMP480 strains couldeffectively express TMV MP dsRNA transcriptsand the dsRNA could protect tobacco from the infection of TMV." @default.
- W2389058082 created "2016-06-24" @default.
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- W2389058082 date "2009-01-01" @default.
- W2389058082 modified "2023-09-25" @default.
- W2389058082 title "KNOCK-OUT THE RNC GENE OF E COLI USING RED-MEDIATED RECOMBINATION FOR CONSTRUCTION OF DSRNA PROKARYOTIC EXPRESSION SYSTEM" @default.
- W2389058082 hasPublicationYear "2009" @default.
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