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- W2389062151 abstract "Rock carp,Procypris rabaudi,an endemic species in China,distributes naturally in the upper reaches of the Yangtze River and in its tributaries.The fish population has been greatly decreased these years due to various factors,such as destruction of spawning conditions,water pollution and over-exploitation.In order to protect the fish population,it is necessary to study the genetic diversity of the fish population.In this study,the mitochondrial DNA(mtDNA) D-loop control region of rock carp collected at Mudong(Md),Fuling(Fl) and Wanzhou(Wz) of the Three Gorges Reservoir was amplified using polymerase chain reaction(PCR) technology,and cloned and sequenced.The results showed that the region fragment was 1 000 bp in length.In a comparative analysis of the mtDNA D-loop region sequences of 45 individuals from the three places,a total of 64 variation sites were detected,including 46 polymorphic sites.The polymorphic sites were 23 for the fish at Md,22 at Fl and 22 at Wz.The average nucleotide difference sites of the fish at the three places were 3.638(at Md),5.410(at Fl) and 4.667(at Wz).The D-loop region sequence divergence of the fish at Mudong,Fuling and Wanzhou was 0.37%,0.56% and 0.49%,respectively.The sequence divergence was 0.42%~0.53% between populations at the three places.A molecular tree was constructed by UPGMA method,and it showed that the individuals at the three sampling places couldn't be clustered into three independent groups in terms of the sampling sites.The little genetic differentiation of the fish at the three sampling sites suggested that individuals of the fish species belong to one single natural population in the Three Gorges Reservoir." @default.
- W2389062151 created "2016-06-24" @default.
- W2389062151 creator A5035843820 @default.
- W2389062151 date "2008-01-01" @default.
- W2389062151 modified "2023-09-23" @default.
- W2389062151 title "Analysis of Genetic Diversity of the Mitochondrial DNA D-Loop Control Region of Rock Carp(Procypris rabaudi(Tchang)) in the Three Gorges Reservoir" @default.
- W2389062151 hasPublicationYear "2008" @default.
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