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- W2389128178 abstract "Objective To establish a double polymerase chain reaction (PCR) assay for rapid detection of Treponema denticola in subgingival plaque specimens, and to explore the association between Treponema denticola infection and severity of chronic periodontal lesion. Methods Subgingival plaque specimens were collected from 158 patients who suffered from chronic periodontitis (CP) with various severity. The specimens were treated with 1% Triton X-100 under 100℃ water bath for 10 minutes to prepare DNA templates. A PCR assay was established to simultaneously detect 16S rDNA of treponema denticola and its tdpA gene that encodes characteristic outer membrane protein. Results Positive rates for 16S rDNA and tdpA gene were 85.4% and 77.2%, respectively, while the negative rate for both was 14.6%. No specimen could be found as positive for tdpA gene but negative for 16S rDNA. The positive detection rates for 16S rDNA and tdpA gene in the specimens were directly correspondent with the CP severity.Conclusions The double PCR assay can be applied to detect rapidly Treponema denticola infection in subgingival plaque specimens of CP. Moreover, the Treponema denticola infection is closely associated with severity of CP." @default.
- W2389128178 created "2016-06-24" @default.
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- W2389128178 date "2004-01-01" @default.
- W2389128178 modified "2023-09-26" @default.
- W2389128178 title "The Association between Treponema Denticola Detected by PCR and the Severity of Periodontitis" @default.
- W2389128178 hasPublicationYear "2004" @default.
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