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- W2389130347 abstract "Microsatellite DNA(SSR) Marker was used to identify both conchocelis and gametophytic blades of the strain of Porphyra haitanensis.Results showed that: A specific band was amplified by 9# primer-pair in conchocelis DNA of the strain,which was significantly different from those in other 14 strains of P.haitanensis and also presented in the gametophytic blades of the strain cultured in the laboratory.When the primer-pair was applied to discriminate blades from the other cultivars in the Porphyra cultivation ground,the SSR marker showed the same results whatever the blades cultured at different cultivation grounds or harvested at different times.Analysis on DNA sequences of the specific bands showed that the SSR markers amplified by 9# primer-pair reflected the changes of simple sequence repeats.Respected motif sequences had been searched out by the software of SSR Hunter and the products were within the prospective lengths when the primer-pair was designed.The sequence turned out to be a new sequence because no similar sequence was found in the NCBI nucleic acid database by BLAST.Analysis of sequences by DNAMAN software showed that there were fewer differences between SF-2 and SF-1 but more differences compared to WT-xp cultivated in Xiapu,Fujian Province.Therefore,it was confirmed that the SSR marker reflected the differences between strains and could be used to identify intra-specific strains.All the above showed that the specific DNA band amplified by 9# primer-pair was the specific SSR marker in both conchocelis and gametophytic blades of the strain and could be used to identify the strain from the current cultivars of P.haitanensis." @default.
- W2389130347 created "2016-06-24" @default.
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- W2389130347 date "2013-01-01" @default.
- W2389130347 modified "2023-09-23" @default.
- W2389130347 title "Research on SSR marker of the SF-2 strain of Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)" @default.
- W2389130347 hasPublicationYear "2013" @default.
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