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- W2389172020 abstract "Aim:To construct the engineered E.coli strain which expresses nattokinase with high expression level.Methods: Both pro-nattokinase gene and nattokinase gene were amplified and cloned into fusion expression vector pJN. Two expression plasmid pJNK1 and pJNK2 were constructed and transferred into BL21(DE3).Results: Both two fusion protein was expressed in a high expression level about 30% of the total cell protein. Preliminary biological assay result indicated the fibroinolytic effect of the induced product from pJNK1(BL) was 2-3 fold stronger than that from pJNK2(BL). Conclusion: Part of the pro-nattokinase fusion protein produced active natokinase through the intramolecular self-processing mechanism. " @default.
- W2389172020 created "2016-06-24" @default.
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- W2389172020 date "2002-01-01" @default.
- W2389172020 modified "2023-10-03" @default.
- W2389172020 title "Molecular clonging and expression of pro-nattokinase gene and nattokinase gene" @default.
- W2389172020 hasPublicationYear "2002" @default.
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