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- W2389175855 abstract "[Objective] The aim was to explore the optimum immobilized conditions of acetylcholinesterase on filter paper coated with chitosan.[Method] Acetylcholinesterase was immobilized on filter paper coated with chitosan using glutaraldehyde as cross-linking reagent,bovine serum albumin as protective agent.And the physical and chemical properties of the immobilized enzyme were studied.[Result] The optimal immobilized conditions were as follows: 1 ml solution was prepared with 50 μl 150 U/ml acetylcholinesterase solution,50 μl 5%(V/V) glutaraldehyde,100 μl 1%(W/W) BSA and 0.2 mol/L phosphate buffer(pH 8.0),and then the filter paper coated with chitosan was immersed for 8 h at 4 ℃.The optimum temperature of the immobilized acetylcholinesterase was 37 ℃,the optimum pH was 8.0,and its could be reused four times.[Conclusion] The study provides a theory basis for rapid determination of organophosphorus pesticide residues in vegetables and fruits." @default.
- W2389175855 created "2016-06-24" @default.
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- W2389175855 date "2012-01-01" @default.
- W2389175855 modified "2023-09-24" @default.
- W2389175855 title "Study on Immobilization of Acetylcholinesterase with Filter Paper Coated with Chitosan" @default.
- W2389175855 hasPublicationYear "2012" @default.
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