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- W2389181750 abstract "Bacillus subtilis is an efficient exogenous protein expression stain which can secrete the target protein into extracellular culture medium.This research clones the gene order of cellulase EGAA from ampullaria gigas gastric juice symbiotic strain Bacillus sp.Strain AC-1 genome and establishes expression vector pAUsp-ega of recombinant bacillus subtilis of cellulase ega using genetic engineering technology.This vector contains a strong promotor and signal peptide and can express exogenous protein with induction by amylum.The enzyme activity of culture medium supernatant obtained by recombinant cellulase ega with CMC-Na as substrate and bacillus subtilis protease deletion form stain WB700 as host bacteria reaches 1120 U/L.This cellulase shows the maximum hydrolysis vigor when pH=6.0;its most appropriate reaction temperature is 60℃.It has a good stability under acidic condition and has a good application prospect." @default.
- W2389181750 created "2016-06-24" @default.
- W2389181750 creator A5010401056 @default.
- W2389181750 date "2013-01-01" @default.
- W2389181750 modified "2023-09-23" @default.
- W2389181750 title "Research on Expression of Cellulase EGA Gene in Bacillus Subtilis and Properties of Its Product" @default.
- W2389181750 hasPublicationYear "2013" @default.
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