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- W2389183713 abstract "Objective To explore the feasibility and effectivity of reconstructed corneal stroma by tissue engineering technique for corneal transplantation.Methods Corneal stroma tissue were reconstructed by coculturing rabbit corneal keratocytes on acellular porcine cornea stromal materials and marking with PKH26 fluorescence dyestuff.Chitosan membrane were transplanted into 16 rabbits corneal stromas and became cornea ulcers and were randomly divided into 2 groups as experimental group and control group.The tissue engineering corneal stromas and xenoma rabbit corneas were transplanted into rabbit ulcerated corneas respectively.Cornea were observed with slit lamp,light microscope and electron microscope.Results The cells in tissue engineering cornea stroma which constructed in vitro were active,its constitution were similar to normal cornea stroma.From the 1st week to the 2nd week after transplantation,the implant's margin of tissue engineering cornea stromas were invasioned by new vessels and became grey and semitransparent;From the 3rd week to the 4th week,new vessels of the implant began to decrescence and parties of it became transparence.After 6 weeks,new vessels of the implant began to decrescence and parties of it became transparence.From the 8th week to the 10th week the cornea ulcers healed and the rabbit corneas became transparent,cornea never regenerated;Ten months,there were no immunological rejection,cornea keep transparent.The therapeutic efficacy of the experimental group were same as the control group.Conclusion Tissue engineering cornea stroma in vitro have no immunological rejection,while they have good histocompatibility,which will be useful transplant material for treating corneal ulcer." @default.
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- W2389183713 date "2006-01-01" @default.
- W2389183713 modified "2023-09-23" @default.
- W2389183713 title "Feasibility and effectivity of reconstructed corneal stroma by tissue engineering technigne for transplantation" @default.
- W2389183713 hasPublicationYear "2006" @default.
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