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- W2389188831 abstract "Objective To investigate the effects of rosiglitazone(Ros) and serum lipid(Lip) on differentiation of ovine subcutaneous and perirenal preadipocytes,and explore the differences in their differentiation in vitro.Method The preadipocytes from fetal lamb subcutaneous and perirenal tissues were cultured,and treated with Ros and(or) Lip at dif-ferent concentrations.The cell differentiation was assessed by changes in activity of the GPDH and quantitation of oil red O staining.PPARγ and LPL mRNA were analyzed by real-time PCR after different inducing treatments.Results Ros and Lip enhanced GPDH activity and increased lipogenesis of preadipocytes,up-regulated mRNA expression of LPL in compari-son with the controls(P 0.05).The optimal inducing concentrations of Ros and Lip for preadipocytes differentiation were 100 nmol /L and 20 μL /mL,respectively.With the optimal inducing concentration,Ros showed more effective on preadi-pocytes differentiation(P 0.05),and significantly up-regulated mRNA expression of PPARγ(P 0.05),while Lip had no significant effect on PPARγ(P 0.05).It showed no significant difference between the effects of single Ros and the combination of Ros and Lip.Preadipocytes from perirenal differentiated less well than that from subcutaneous tissue when exposed to the same differentiation-inducing media(P 0.05).Conclusions The results of this study suggest that Ros and Lip induce differentiation of ovine preadipocytes in vitro.With the same inducing media,preadipocytes from perirenal differentiate less well than that from subcutaneous tissue." @default.
- W2389188831 created "2016-06-24" @default.
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- W2389188831 date "2011-01-01" @default.
- W2389188831 modified "2023-09-25" @default.
- W2389188831 title "Effects of rosiglitazone and serum lipid on differentiation of ovine preadipocytes" @default.
- W2389188831 hasPublicationYear "2011" @default.
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