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- W2389200042 abstract "Objective To investigate the effects and mechanisms of rheumatoid fibroblast-like synoviocytes(FLS) in promoting osteoclastogenesis.Methods FLS were separated and cultured from synovium tissue of patients with active rheumatoid arthritis(RA) or osteoarthritis(OA).FLS were then cocultured with normal monocytes from healthy volunteers' peripheral blood.Osteoclasts were determined and counted by trate-resistant acid phosphatase(TRAP) staining,and resorption lacunae on bone slices were identified by toluidine blue staining.The expression of receptor activator of NF-κB ligand(RANKL) on FLS was evaluated by immunofluorescence.Real-time PCR and Western blot were performed respectively to determine the mRNA and protein expression of RANKL and osteoprotegerin(OPG).Results At the 7th day of cocultured RA-FLS with normal monocytes,few TRAP-positive cells with three or more nuclei were seen and these osteoclasts increased significantly by the 14th day.Resorption lacunae were observed at the 21st day.However,no osteoclast or resorption lacuna was observed when OA-FLS was cocultured with normal monocytes.Immunofluorescence staining showed significantly higher expression of RANKL in RA-FLS than in OA-FLS(P0.05).Expression of RANKL mRNA and protein was significantly up-regulated in RA-FLS compared with that in OA-FLS,while expression of OPG mRNA and protein was significantly down-regulated.The ratio of RANKL/OPG mRNA and protein was also up-regulated in RA-FLS more obviously than in OA-FLS(all P0.05).Conclusion RA-FLS may promote osteoclastogenesis and bone erosion in RA by RANKL up-regulation." @default.
- W2389200042 created "2016-06-24" @default.
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- W2389200042 date "2011-01-01" @default.
- W2389200042 modified "2023-09-25" @default.
- W2389200042 title "Rheumatoid synovial fibroblasts promote osteoclastogenesis via RANKL" @default.
- W2389200042 hasPublicationYear "2011" @default.
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