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- W2389240026 abstract "AIM: To achieve the soluble expression of an immnotoxin consisting of an anti hepatoma disulfide stabilized scFv and truncated pseudomonas exotoxin (PE38). METHODS: dsFv PE38 fusion gene was inserted into two prokaryotic expression vectors, which contain the GST and Trx gene respectively, and was transformed into different host strains. The concentration of IPTG, time and temperature were adjusted and the specificity of the dsFv PE38 fusion protein was examined by ELISA. RESULTS: The fusion protein with M r 66 000 was successfully expressed in E.coli Origami (DE3) in soluble supernatant, which amounted to 21% of the total soluble proteins. ELISA showed that, like its parent disulfide stabilized scFv, dsFv PE38 had similar specificity to the hepatoma cells. CONCLUSION: The immunotoxin dsFv PE38 is successfully expressed in soluble supernatant, which will provide a new way to the study of soluble expression of other fusion proteins." @default.
- W2389240026 created "2016-06-24" @default.
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- W2389240026 date "2003-01-01" @default.
- W2389240026 modified "2023-09-24" @default.
- W2389240026 title "Soluble expression of anti-hepatoma dsFv-PE38 fusion protein in E.coli" @default.
- W2389240026 hasPublicationYear "2003" @default.
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