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- W2389248990 abstract "Objective Study on transfer efficiency in human tumor cell lines by liposome mediated TGF-β1 shRNA and provides a possible research method of RNA interference to explore pathogenic role of TGF-β1 signaling pathway in tumor microenvironment. Methods Chemically synthetic siRAN against the TGF-β1 protein, combined with LipofectamineTM2000 to prepare Lip-shRNA complexes. After exposure to the different dose of complexes for 24 hours, the fluorescence indexes of RD cells were analyzed by flow cytometry. The transfer rates were assessed by manual counting under fluorescent microscope. The TGF-β1 protein expression of cells transferred for different time was inspected by immunofluorescent. Results The fluorescent microscope showed that the fluorescent phosphorothioated oligonucleotide located in cytoplasm of RD cells after transferred for 4 h, and the shRNA shifted to nucleus of RD cells after 8 h. The transfer rate examined by FCM showed compellent dependent correlation with the doses of Lip-shRNA complexes. The transfer rate by manual counting were dependently correlated with the lower doses of Lip-shRNA complexes, but didn't increase obviously with the high dose of complexes. The TGF-β1 protein expression of RD cells transferred for 6 days is decreased more than that of RD cells transferred for 3 days, and there were significant difference(P0.05) compare to the control group. Conclusion The transfer efficiency of TGF-β1 shRNA mediated by liposome in tumor cells depends on stability of oligonucleotide, dose of Lip-shRNA complexes, and transfer time,this technology may used as a way of research to explore pathogenic role of TGF-β1 signaling pathway in tumor microenvironment." @default.
- W2389248990 created "2016-06-24" @default.
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- W2389248990 date "2008-01-01" @default.
- W2389248990 modified "2023-09-25" @default.
- W2389248990 title "The Method of TGF-β1 shRNA Transfection Efficiency Mediated by Liposomes" @default.
- W2389248990 hasPublicationYear "2008" @default.
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