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- W2389266695 abstract "Objective To investigate the effect of Adenovirus-mediated averse vascular endothelial growth factor_ 165(Ad-aVEGF_ 165)on the growth of human melanoma cells(A_ 375) in vivo and in vitro. Methods In vitro, the 100 multiplicity of infection of Aadenovirus- mediated green fluorescent protein(Ad-GFP)and Ad-aVEGF_ 165 were transfected into human endothelium cell of vessel_ 304(ECV_ 304) and A_ 375. ECV_ 304 cells were divided into 3 groups: A_ 375 group, Ad-GFP group and Ad-aVEGF_ 165 group. A_ 375 cells were also divided into 3 groups: 1640 group, Ad-GFP group and Ad-aVEGF_ 165 group. Their effects were analyzed by proliferation assay, cell cycle, and VEGF expression. In vivo, A_ 375 cells were injected into the axilla of the nude mouse. When the tumor formed, they were transplanted into another 15 mice. After treatment, the tumor was excised for naked eye observation, HE observation and micro-vascular density(MVD) counting. Results The cell supernatant fluid of A_ 375 group and Ad-GFP group could stimulate ECV_ 304 cell growth,but that of Ad-aVEGF_ 165 group could inhibit the growth of ECV_ 304 cell. All the A_ 375 cells in 3 groups had the proliferation trend, showing no statistically significant difference(P0.05). ECV_ 304 cell proliferation index(PI) in Ad-aVEGF_ 165 group reduced(P0.05). There was no statistically significant difference(P0.05) in the PI of A_ 375 cell. The A_ 375 cell integral optical densities were 234.41±13.8 in 1640 group, 222.73±3.67 in Ad-GFP group and 180.84±6.34 in Ad-aVEGF_ 165 group. The tumor volume in Ad-aVEGF_ 165 group was smaller than that in Ad-GFP group and PBS group at 2 weeks after operation, the trend became much obvious with the time delay. Ad-aVEGF_ 165 brought to much tissue necrosis under HE stain. The MVD of PBS group, Ad-GFP group and Ad-aVEGF_ 165 group were 65±10view,52±11view and 30±6view, respectively. Conclusion In Vitro, Ad-VEGF_ 165 gene could inhibited ECV_ 304 cells' growth by weakening VEGF expression of A_ 375 cells. In vivo, Ad-aVEGF_ 165 could inhibit the growth of human melanoma from blocking micro-vascular." @default.
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- W2389266695 date "2006-05-01" @default.
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- W2389266695 title "[Experimental studies on inhibition of malignant melanoma growth by gene transfer of adenovirus-mediated averse vascular endothelial growth factor165]." @default.
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