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- W2389295573 abstract "Aim To explore the possible mechanism of Rheum tanguticum polysaccharide (RTP) in repairing mucosal membrane by observing its effects on the proliferation, differentiation and migration in normal human intestinal epithelial cell line (HIEC) in vitro.Methods 24 h after cell inoculation, RTP were added into the cultured media with different concentrations. MTT assay was used to observe the effect of RTP on cell proliferation, while HE staining and alkaline phosphatase (ALP) activity measurement were introduced to assess cell differentiation. After the cultured HIEC cells formed a complete monolayer,an artificial wound with a cell free area of constant size was made in the center of the mucosal cell sheet. RTP were added just after artificial wounding and its effects on cell migration were monitored under phase-contrast microscopy and photographs were taken every 12 h for 48 h after wounding. And changes in the cell-free area over time were quantified by using an image analyzer.Results RTP dose-dependently enhanced the proliferation, migration and differentiation of HIEC cells. Compared with Normal group, there existed significant differences on cell proliferation, migration and ALP activity(P0.05 or P0.01).Conclusion RTP might improve intestinal mucosal repair by enhancing intestinal epithelial cell proliferation, migration and differentiation." @default.
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- W2389295573 date "2005-01-01" @default.
- W2389295573 modified "2023-09-24" @default.
- W2389295573 title "Effects of Rheum tanguticum polysaccharide in promoting HIE Ccell proliferation, differentiation and migration" @default.
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