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- W2389321331 abstract "The upstream open reading frames(uORF)in 5'-untranslated regions (5'-UTR)of eukaryotic mRNAs play an important role in translation efficiency.The computer analysis of upstream ATG and uORF of 5'-UTR of plants mRNA adopted from the nucleotide sequence databank revealed that up to 18% of 5'-UTRs contained uATG,much more than the previous estimate.A- mong them,about 50% of genes had one uATG and nearly 20% had two uATG.About 85% of uORFs were non-overlapping,and nearly 80% of them fell in the range of below 20 aa (30% of uORF peptides were 1～5 aa).Sequences flanking the uATG codon strik- ingly differed from the functional initiation codons and the upstream ATG triplets were more frequently located in a non-optimal con- text.Consensus sequences of the ATG codon contexts of mRNA with and without uATG were similar but the ATG codon contexts of mRNA without uATG were more frequently located in an optimal context than that of mRNA with uATG.Most of mRNA with uATG possibly had relation with the regulatory function.In addition,most of uORFs had no similiarity between plant mRNA species,where- as sequences of a few uORFs were highly conserved,such as uORFs of S-Adenosyl-L-methionine decarboxylase(AdoMet-DC)were 75%～100% homology between plant mRNA species,which were much more conserved than AdoMetDC." @default.
- W2389321331 created "2016-06-24" @default.
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- W2389321331 date "2004-01-01" @default.
- W2389321331 modified "2023-09-23" @default.
- W2389321331 title "Upstream Open Reading Frames (uORF) Analysis of Plant mRNAs" @default.
- W2389321331 hasPublicationYear "2004" @default.
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