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- W2389329587 abstract "Aim To establish a sensitive biotin strepavidin double antibody sandwich ELISA for mouse IL 5,IL 4,IFN γ and to explore its application in IL-5 transgenic mice infected with Nippostrongylus brasiliensis.Method The culture supernatants of hybridoma were purified by ultrafiltering with PM10 membrane,precipitating with 50% of saturated ammonium sulfate and Protein G Sepharose affinity column.Purified TRFK4,BVD6 and AN18 McAbs were biotinylated with the biotinylation reagent.2μg/ml of TRFK5,8μg/ml of 11B11 and 4μg/ml of R4-6A2 were as coating antibodies of detecting IL-5,IL-4 and IFN-γ,separately,Then the samples,biotinylated TRFK4(1μg/ml),BVD6(2μg/ml) and AN18(1μg/ml),SA HRPO and OPD were added.Finally,the reaction was stopped by sulfuric acid and A 490 value was read. Results The method had a high specificity and sensitivity.The promising results have been obtained for detecting the levels of cytokines of sera from IL 5 transgenic mice and non transgenic mice infected with N.brasiliensis.Conclusion The method can be used to detect mouse cytokines." @default.
- W2389329587 created "2016-06-24" @default.
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- W2389329587 date "2002-01-01" @default.
- W2389329587 modified "2023-09-23" @default.
- W2389329587 title "Establishment of double antibody sandwich ELISA for mouse cytokines and its application" @default.
- W2389329587 hasPublicationYear "2002" @default.
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