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- W2389332561 abstract "To isolate the collagen phagocytic subpopulation of fibroblast (CPSF) and non-collagen phagocytic subpopulation of fibroblast (nCPSF) and to identify their differentially expressed genes. The CPSF and nCPSF was isolated by using collagen-fluorescein-isothiocynate-latex bead (COL-FITC-LB) phagocytosis technique and FCM sorting method. Microarray analysis was used to screen the differentially expressed genes, which were verified by real-time PCR. CPSF and nCPSF was successfully isolated. Seventeen differentially expressed genes were identified. Compared with nCPSF, the expression of 12 or 5 genes was up-regulated or down-regulated in CPSF. Three of the 12 up-regulated genes were urokinase plasminogen activator receptor-associated protein (uPARAP), cytochrome b-245, beta polypeptide (CYBB) and Hook homolog 1 (HOOK1), which were confirmed by real-time PCR. uPARAP mRNA expression level in CPSF was 2788 times of that in nCPSF. CYBB mRNA expression in CPSF was only 0.85 times of that in nCPSF. HOOK1 mRNA expression in CPSF was 1.96 times of that in nCPSF (P<0.05). A novel method is successfully established to isolate CPSF and nCPSF. uPARAP is the main differentially expressed gene in CPSF and nCPSF, which is obviously involved in the fibroblast collagen phagocytosis. It might be a potential biomarker for treatment of collagen diseases.目的：探讨建立新的分离人成纤维细胞胶原吞噬亚群(CPSF)和成纤维细胞非胶原吞噬亚群(nCPSF)的方法，并筛选两者差异表达基因。方法：根据CPSF和nCPSF吞噬功能的差异，联合应用胶原包被的异硫氰酸荧光乳胶微球(COL-FITC-LB)吞噬技术和流式细胞分离技术分离二者，并通过生物芯片技术和real-time PCR筛选、验证两者差异表达基因，寻找CPSF和nCPSF具有特征性的分子标志物。结果：成功分离CPSF和nCPSF，并对其基因差异性分析，得到17个差异表达基因。与nCPSF比较，CPSF中有12个基因表达上调，5个表达下调。对其中与胶原吞噬功能可能相关的3个基因进行验证，即纤溶酶原激活物受体相关蛋白(urokinase plasminogen activator receptor-associated protein，uPARAP)、细胞色素b-245，β多肽(cytochrome b-245, beta polypeptide，CYBB)和同源物1(Hook homolog 1，HOOK1)，CPSF中uPARAP表达量为nCPSF的2 788倍；CPSF中CYBB表达量为nCPSF的0.85倍；CPSF中HOOK1表达量为nCPSF的1.96倍，差异具有统计学意义(P<0.05)。结论：建立了分离CPSF和nCPSF的新方法。CPSF和nCPSF最主要的差异表达基因是uPARAP，对成纤维细胞胶原吞噬功能具有重要影响，是治疗胶原代谢疾病新的候选靶分子之一。." @default.
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- W2389332561 date "2015-06-01" @default.
- W2389332561 modified "2023-09-23" @default.
- W2389332561 title "[Isolation and gene screening for collagen phagocytic subpopulation of fibroblasts and non-collagen phagocytic subpopulation of fibroblasts]." @default.
- W2389332561 doi "https://doi.org/10.11817/j.issn.1672-7347.2015.06.006" @default.
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