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- W2389347768 abstract "To prepare monoclonal antibodies(mAb) against LAIR-2 and establish sandwich ELISA to detect LAIR-2,distribution of LAIR-2 was investigated with indirect fluorescence staining and flow cytomytry analysis. Based on epitope mapping of LAIR-2 and LAIR-2 mAb HRP-conjugation, the sandwich ELISA detecting LAIR-2 was established. The potential role played by LAIR-2 mAb 3G4 in cytotoxicity function was identified with redirected cytotoxicity assay(RCA). Four strains of mAb recognizing LAIR-2-myc fusion protein were obtained by routine hybridoma technique. Among the four strains of mAb, one clone named 3G4 can recognize both LAIR-1 and LAIR-2, but it couldn't inhibit the cytotoxicity induced by CD16 mAb in RCA. The limitation measured by sandwich ELISA for detecting LAIR-2 reached to 5 ng/ml, which may be useful for basic and clinical research on LAIR-2 distribution and quantitative analysis." @default.
- W2389347768 created "2016-06-24" @default.
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- W2389347768 date "2004-01-01" @default.
- W2389347768 modified "2023-09-27" @default.
- W2389347768 title "Preparation and Identification of Monoclonal Antibodies Against Leukocyte-Associated Immunoglobulin-Like Receptor-2 (LAIR-2) and Establishment of Sandwich ELISA for Detecting LAIR-2" @default.
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