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- W2389352178 abstract "Objective:To obtain the full-long genomic sequence of the subtilisin-like protease (Pr1) gene of Pythium carolinianum. Methods: A mini genomic DNA library was constructed. Firstly, the genomic DNA was digested with Xho I and then inserted into the pBluescript SK(-) plasmid which was digested with the same enzyme and treated with calf intestinal alkaline phosphatase. The recombinant plasmid was transferred into Ecoli JM109 and the mini genomic DNA library was constructed. Results: A 780bp PCR product was subsequently subcloned with nested PCR method with T3 promoter primer and nested primers S1 and S2. Sequence analysis indicated that it contains the 301bp unknown sequences adjacent to the known sequence of Pr1 gene of P. carolinianum. Conclusion: Constructing mini genomic DNA library is an efficient and expeditious strategy of searching for unknown sequence adjacent to the known." @default.
- W2389352178 created "2016-06-24" @default.
- W2389352178 creator A5016279165 @default.
- W2389352178 date "2005-01-01" @default.
- W2389352178 modified "2023-09-23" @default.
- W2389352178 title "Cloning of the Unknown Sequence of Subtilisin-like Protease Gene in Pythium carolinianum by Constructing Mini Genomic DNA Library" @default.
- W2389352178 hasPublicationYear "2005" @default.
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