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- W2389369311 abstract "Objective:To establish a method to determine Quercetin,Kaempferol and Isorhamnetin in Rhizoma alpiniae officinarum.Methods:A HPLC method was used.Chromatographic assay was performed on a Kromasil C18(5μm,4.6mm×250mm)column.The mobile phase consisted of methanol-0.4% phosphoric(52:48),the detection wavelength was set at 360nm.Results:The linear range of Quercetin concentration was from 0.962 to 24.05μg/ mL and correlation coefficient was 0.99994.The linear range of Kaempferol concentration was from 1.008 to 25.2g/mL and correlation coefficient was 0.99990.The linear range of Isorhamnetin concentration was from 0.983 to 24.575 g/ mL and correlation coefficient was 0.99996.The average recovery of Quercetin was 97.97% and RSD was 1.59%(n=6).The average recovery of Kaempferol was 97.35% and RSD was 1.86%(n=6).The average recovery of Isorhamnetin was 98.11% and RSD was 2.37%(n=6).Conclusion:The method is simple,accurate and reproducible for the determination of Quercetin,Kaempferol and Isorhamnetin in Rhizoma alpiniae officinarum." @default.
- W2389369311 created "2016-06-24" @default.
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- W2389369311 date "2010-01-01" @default.
- W2389369311 modified "2023-09-24" @default.
- W2389369311 title "Determination of Quercetin Kaempferol and Isorhamnetin in Rhizoma Alpiniae Officinarum by HPLC" @default.
- W2389369311 hasPublicationYear "2010" @default.
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