Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389383566> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2389383566 abstract "AIM: To investigate the effect of targeting gene therapy on mouse SiHa cell cervical carcinoma by transfecting siRNA into the tumor with solid-phase method in vivo. METHODS: A sense strand siRNA(21 nt) for human papilloma virus type 16(HPV16) was designed.siRNA-Lipo2000-carbomer gum was prepared.Forty SCID mice with SiHa cell cervical carcinoma were divided into experimental group(n=32) and control group(n=8).The diameter and volume of the tumors were measured before treatment.The mice in experimental group were treated with siRNA-Lipo 2000-carbomer gum for 7 d.The control mice were treated with Lipo 2000-carbomer gum for 7 d.The mice in experimental group and control group were sacrificed at the time points of 4 d,8 d,12 d and 16 d after treatment.The diameter and volume of the tumors were measured again.The HPV16-DNA in the tumor tissues was measured by PCR.The protein levels of P16 and P53 in the tumors were determined by the method of immunohistochemistry. RESULTS: Compared with control group,the titer of HPV16-DNA decreased significantly at the time points of 8 d and 12 d after transfection in experimental group(P0.05).The protein expression of P16 in experimental group showed decreased tendency 8 d and 12 d after transfection,but without statistical difference.The protein expression of P53 significantly decreased 8 d and 12 d after transfection.The tumor volume in experimental group was significantly decreased as compared to that in control group at the time point of 12 d(P0.05).Transfection of siRNA for 12 d resulted in attenuating dyskaryosis and karyoplasmic ratio of the tumor cells.CONCLUSION: Solid-phase transfaction of siRNA in vivo inhibits HPV-DNA replication and growth of SiHa cell cervical carcinoma." @default.
- W2389383566 created "2016-06-24" @default.
- W2389383566 creator A5001561621 @default.
- W2389383566 date "2011-01-01" @default.
- W2389383566 modified "2023-09-27" @default.
- W2389383566 title "siRNA inhibits HPV16-DNA replication,tumor volume and expression of P16 and P53 in SCID mice with SiHa cell cervical carcinoma in vivo" @default.
- W2389383566 hasPublicationYear "2011" @default.
- W2389383566 type Work @default.
- W2389383566 sameAs 2389383566 @default.
- W2389383566 citedByCount "0" @default.
- W2389383566 crossrefType "journal-article" @default.
- W2389383566 hasAuthorship W2389383566A5001561621 @default.
- W2389383566 hasConcept C104317684 @default.
- W2389383566 hasConcept C111599444 @default.
- W2389383566 hasConcept C142724271 @default.
- W2389383566 hasConcept C1491633281 @default.
- W2389383566 hasConcept C150903083 @default.
- W2389383566 hasConcept C153911025 @default.
- W2389383566 hasConcept C185592680 @default.
- W2389383566 hasConcept C204232928 @default.
- W2389383566 hasConcept C207001950 @default.
- W2389383566 hasConcept C2777546739 @default.
- W2389383566 hasConcept C502942594 @default.
- W2389383566 hasConcept C54009773 @default.
- W2389383566 hasConcept C54355233 @default.
- W2389383566 hasConcept C55493867 @default.
- W2389383566 hasConcept C71924100 @default.
- W2389383566 hasConcept C81885089 @default.
- W2389383566 hasConcept C86803240 @default.
- W2389383566 hasConceptScore W2389383566C104317684 @default.
- W2389383566 hasConceptScore W2389383566C111599444 @default.
- W2389383566 hasConceptScore W2389383566C142724271 @default.
- W2389383566 hasConceptScore W2389383566C1491633281 @default.
- W2389383566 hasConceptScore W2389383566C150903083 @default.
- W2389383566 hasConceptScore W2389383566C153911025 @default.
- W2389383566 hasConceptScore W2389383566C185592680 @default.
- W2389383566 hasConceptScore W2389383566C204232928 @default.
- W2389383566 hasConceptScore W2389383566C207001950 @default.
- W2389383566 hasConceptScore W2389383566C2777546739 @default.
- W2389383566 hasConceptScore W2389383566C502942594 @default.
- W2389383566 hasConceptScore W2389383566C54009773 @default.
- W2389383566 hasConceptScore W2389383566C54355233 @default.
- W2389383566 hasConceptScore W2389383566C55493867 @default.
- W2389383566 hasConceptScore W2389383566C71924100 @default.
- W2389383566 hasConceptScore W2389383566C81885089 @default.
- W2389383566 hasConceptScore W2389383566C86803240 @default.
- W2389383566 hasLocation W23893835661 @default.
- W2389383566 hasOpenAccess W2389383566 @default.
- W2389383566 hasPrimaryLocation W23893835661 @default.
- W2389383566 hasRelatedWork W2139279290 @default.
- W2389383566 hasRelatedWork W2230893852 @default.
- W2389383566 hasRelatedWork W2348413630 @default.
- W2389383566 hasRelatedWork W2349027243 @default.
- W2389383566 hasRelatedWork W2350697797 @default.
- W2389383566 hasRelatedWork W2352735759 @default.
- W2389383566 hasRelatedWork W2357696120 @default.
- W2389383566 hasRelatedWork W2367637364 @default.
- W2389383566 hasRelatedWork W2369945747 @default.
- W2389383566 hasRelatedWork W2371436664 @default.
- W2389383566 hasRelatedWork W2380848180 @default.
- W2389383566 hasRelatedWork W2383777926 @default.
- W2389383566 hasRelatedWork W2385415743 @default.
- W2389383566 hasRelatedWork W2386129233 @default.
- W2389383566 hasRelatedWork W2392166498 @default.
- W2389383566 hasRelatedWork W2558145881 @default.
- W2389383566 hasRelatedWork W3028682169 @default.
- W2389383566 hasRelatedWork W3029436396 @default.
- W2389383566 hasRelatedWork W3030632957 @default.
- W2389383566 hasRelatedWork W2605684978 @default.
- W2389383566 isParatext "false" @default.
- W2389383566 isRetracted "false" @default.
- W2389383566 magId "2389383566" @default.
- W2389383566 workType "article" @default.