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- W2389400982 abstract "Objective To investigate the damage to cultured cells caused by respiratory syncytial virus (RSV) and human cytomegalovirus (HCMV) coinfection. Method MTT colorimetric assay was used to measure the A value of the following four WI 38 cell samples, including cells which were not infected with viruses, infected with RSV, infected with RSV and HCMV simultaneously, cells coinfected with HCMV 12 hours after RSV infection, there were altogether 80 samples, 20 samples per group. The cell cycle progression of the following six WI 38 cell samples were investigated by using flow cytometry, including the normal cells, cells infected with RSV, cells infected with HCMV, cells coinfected with RSV and HCMV simultaneously, cells coinfected with HCMV 12 hours after RSV infection and cells coinfected with RSV 12 hours after HCMV infection, there were altogether 72 samples, 12 samples per group. Results (1) The A value of normal cell samples (1.50±0.12) was greater than that of cells infected with RSV (1.12±0.13) and coinfected with RSV and HCMV (1.09±0.08) ( P 0.01). The A value of RSV infected cells was not significantly different from that of RSV and HCMV coinfected cells ( P 0.05). (2) The proportion of S phase cell of normal cells (15.31±1.08) was higher than that of cells infected with virus ( P 0.01), and the proportion of S phase cell of RSV infected cells (10.65±0.72) was higher than that of HCMV infected cells (5.65±1.35) and RSV and HCMV coinfected cells (5.02±1.08, 4.79±0.28, 5.14± 0.56) ( P 0.01). No significant difference was found in the proportion between HCMV infected cells and RSV and HCMV coinfected cells ( P 0.05). Conclusion The numbers of living cells were not different between RSV infected cells and RSV and HCMV coinfection. The cell cycle was inhibited by HCMV and RSV infection, but the inhibition induced by single HCMV infection and coinfection with RSV and HCMV was stronger than that by single RSV infection." @default.
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- W2389400982 title "The damage to cultured cells caused by respiratory syncytial virus and human cytomegalovirus coinfection" @default.
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