Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389408057> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2389408057 abstract "Objective: To transfer the human beta-nerve growth factor gene recombinant adenovirus vector (Ad-hNGFβ) into rat spinal cord astrocyte in vitro and to examine whether it could deliver potent peptides in the nervous system and observe the cell toxic action as well. Methods: The astrocytes from neogenesis SD rat spinal cord were mechanically dissociated, and the DMEM/F-12 medium was adopted to primary culture and passaged the cells, and then identified by GFAP (glial fibrillary acidic protein) immumofluorescence method. The recombinant Ad-hNGFβ was transfected into AST cells at an MOI of 25, 50 and 100 respectively, and the toxicity of Ad-hNGFβ to astrocyte was analyzed by MTT method. The expression of NGFβ were identified by ELISA in astrocyte cultured supernatant at 3, 6, 9, 12 and 15 d respectively. The capacity to stimulate the proliferation and the apoptosis ratio was determined by flow cytometry. Relative molecular weight was measured to determine the construction by Western blot. Results: The astrocytes from neogenesis SD rat spinal cord were successfully cultured, which showed positive activity to GFAP. Recombinant adenovirus vector had no obvious toxicity to astrocytes at multiplicity of infection(MOI)at 25, 50 and 100. The expression of hNGFβ were significant differet between control group and testing group(P0.01); the hNGFβ expression were gradually decreased with the extension of time. The expressed hNGFβ had the capacity to stimulate the proliferation of astrocytes. The construction of expressed protein is dimeric of hNGFβ. Conclusion: The spinal cord astrocytes can be infected directly by the recombinant adenovirus containing hNGFβ gene and express biologically active protein efficiently. Recombinant adenovirus vector has no obvious cell toxic effect and it could release beta-NGF to elevate the cytoactive at a suitable MOI." @default.
- W2389408057 created "2016-06-24" @default.
- W2389408057 creator A5069247523 @default.
- W2389408057 date "2007-01-01" @default.
- W2389408057 modified "2023-09-25" @default.
- W2389408057 title "Infection of Astrocyte from Rat Spinal Cord with Recombinant Adenovirus Containing Human NGF-beta Gene and the Biological Activity Research" @default.
- W2389408057 hasPublicationYear "2007" @default.
- W2389408057 type Work @default.
- W2389408057 sameAs 2389408057 @default.
- W2389408057 citedByCount "0" @default.
- W2389408057 crossrefType "journal-article" @default.
- W2389408057 hasAuthorship W2389408057A5069247523 @default.
- W2389408057 hasConcept C104317684 @default.
- W2389408057 hasConcept C113246987 @default.
- W2389408057 hasConcept C134018914 @default.
- W2389408057 hasConcept C153911025 @default.
- W2389408057 hasConcept C169760540 @default.
- W2389408057 hasConcept C170493617 @default.
- W2389408057 hasConcept C202751555 @default.
- W2389408057 hasConcept C203014093 @default.
- W2389408057 hasConcept C203233990 @default.
- W2389408057 hasConcept C204232928 @default.
- W2389408057 hasConcept C2777542381 @default.
- W2389408057 hasConcept C2778423431 @default.
- W2389408057 hasConcept C2780495277 @default.
- W2389408057 hasConcept C2780775167 @default.
- W2389408057 hasConcept C40767141 @default.
- W2389408057 hasConcept C529278444 @default.
- W2389408057 hasConcept C54009773 @default.
- W2389408057 hasConcept C54355233 @default.
- W2389408057 hasConcept C55493867 @default.
- W2389408057 hasConcept C81885089 @default.
- W2389408057 hasConcept C86803240 @default.
- W2389408057 hasConceptScore W2389408057C104317684 @default.
- W2389408057 hasConceptScore W2389408057C113246987 @default.
- W2389408057 hasConceptScore W2389408057C134018914 @default.
- W2389408057 hasConceptScore W2389408057C153911025 @default.
- W2389408057 hasConceptScore W2389408057C169760540 @default.
- W2389408057 hasConceptScore W2389408057C170493617 @default.
- W2389408057 hasConceptScore W2389408057C202751555 @default.
- W2389408057 hasConceptScore W2389408057C203014093 @default.
- W2389408057 hasConceptScore W2389408057C203233990 @default.
- W2389408057 hasConceptScore W2389408057C204232928 @default.
- W2389408057 hasConceptScore W2389408057C2777542381 @default.
- W2389408057 hasConceptScore W2389408057C2778423431 @default.
- W2389408057 hasConceptScore W2389408057C2780495277 @default.
- W2389408057 hasConceptScore W2389408057C2780775167 @default.
- W2389408057 hasConceptScore W2389408057C40767141 @default.
- W2389408057 hasConceptScore W2389408057C529278444 @default.
- W2389408057 hasConceptScore W2389408057C54009773 @default.
- W2389408057 hasConceptScore W2389408057C54355233 @default.
- W2389408057 hasConceptScore W2389408057C55493867 @default.
- W2389408057 hasConceptScore W2389408057C81885089 @default.
- W2389408057 hasConceptScore W2389408057C86803240 @default.
- W2389408057 hasLocation W23894080571 @default.
- W2389408057 hasOpenAccess W2389408057 @default.
- W2389408057 hasPrimaryLocation W23894080571 @default.
- W2389408057 hasRelatedWork W2004705786 @default.
- W2389408057 hasRelatedWork W2135787392 @default.
- W2389408057 hasRelatedWork W2274029474 @default.
- W2389408057 hasRelatedWork W2348967180 @default.
- W2389408057 hasRelatedWork W2352423340 @default.
- W2389408057 hasRelatedWork W2354516331 @default.
- W2389408057 hasRelatedWork W2364400368 @default.
- W2389408057 hasRelatedWork W2367530409 @default.
- W2389408057 hasRelatedWork W2369176630 @default.
- W2389408057 hasRelatedWork W2369441023 @default.
- W2389408057 hasRelatedWork W2370240086 @default.
- W2389408057 hasRelatedWork W2370874833 @default.
- W2389408057 hasRelatedWork W2373440300 @default.
- W2389408057 hasRelatedWork W2375901358 @default.
- W2389408057 hasRelatedWork W2377561258 @default.
- W2389408057 hasRelatedWork W2377918449 @default.
- W2389408057 hasRelatedWork W2386280826 @default.
- W2389408057 hasRelatedWork W2390274409 @default.
- W2389408057 hasRelatedWork W2392821486 @default.
- W2389408057 hasRelatedWork W2436702581 @default.
- W2389408057 isParatext "false" @default.
- W2389408057 isRetracted "false" @default.
- W2389408057 magId "2389408057" @default.
- W2389408057 workType "article" @default.