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- W2389442325 abstract "Objective To analyze the drug-resistance β-lactamse in Acinetobacter and investigate effects of AmpR and AmpD gene of AmpC β-lactamase on producing enzyme by Acinetobacter. Methods AmpC β-lactamase of 126 acinetobacter strains were detected by Nitrocefin development process,cefoxitin disk diffusion method and three-dimensional test. Plasmid DNA and genomic DNA were amplified and sequenced using AmpC gene primer PCR strategy,then AmpR and AmpD genes of positive strains were amplified. Result The β-lactamase ratio was 78.6%. Thirty-eight isolates were confirmed to produce AmpC β-lactamases by the three-dimensional extract test and PCR amplification of AmpC structure gene.The AmpC gene consisted in the chromosome and not in the plasmids. Nineteen isolates possessed the AmpD gene. Thirty-three isolates possessed the AmpR gene. Conclusion The AmpC gene consist in the chromosome and not in the plasmid. The AmpD and AmpR gene mutants maybe result in overexpression of AmpC β-lactamases. The three-dimensional extract test to detect the AmpC β-lactamase have high false positive and low false negative rates." @default.
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- W2389442325 date "2007-01-01" @default.
- W2389442325 modified "2023-09-25" @default.
- W2389442325 title "AmpC β-lactamase gene in Acinetobacter and its gene analysis" @default.
- W2389442325 hasPublicationYear "2007" @default.
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