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- W2389444476 abstract "Aim: To observe the effects of different dose of GM-1 on the growth and differentiation of neural stem cells. Methods; The neural stem cells were isolated from human embryonic brains and cultured in serum-free medium-DMEM/F12 with bFGP' and B27 ,Different dosage of GM-1 was added into the different experimental group,and the proliferation ability of the cells were measured by MTT methods. The differentiation of the cells was induced in the medium-DMEM/F12 with 3% serum and observed through inverted phase-contrast microscope every 6 hours. Results: According to t test,the 5 d and 10 d MTT showed as follows: there was no significant difference between bFGF(20 μg/L) + B27 + DMEM/F12 and GM-1(33.5 μg/L) + B27 + DMEM/F12, however,the significant difference lay in GM-1 (33. 5 μg/ml) + B27 + DMEM/F12 and B27 + DMEM/F12, there was no significant differences among GM-1 (0. 335 μg/L) + B27 + DMEM/F12,GM-1(3.35 μg/L) + B27 + DMEM/F12 and B27 + DMEM/F12. The differential experiments showed that: the neurites from the neuralspheres of (3% )serum + DMEM/F12 group stuck in a radial way after the neural stem cells being replanted in the 24-well plate coated with poly-lysine for six hours while the neurites of the three experimental groups and the negative-control group were short and thin, the neurites of each group developed little by little with the time gone by,however, the neurites of the three experimental groups and the negative-control group were much less than those of positive-control group. Conclusion: GM-1 is able to maintain the primitive situation of neural stem cell and has effects on its growth, however,it has no obvious influence on its differentiation." @default.
- W2389444476 created "2016-06-24" @default.
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- W2389444476 date "2003-01-01" @default.
- W2389444476 modified "2023-09-24" @default.
- W2389444476 title "Influence of GM-1 on the proliferation and differentiation of neural stem cells" @default.
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