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- W2389474126 abstract "Objective:To investigate the osteogenic potential of the osteoblasts induced from human umbilical cord blood(HUCB) and adult bone marrow MSCs.Method:The mononuclear fraction was isolated using standard Ficoll Hypaque techniques. Both kinds of cells were purified,proliferated and cultured in the same culture medium.Dexamethasone,ascorbic acid 2 phosphate and β glycerophosphate were used for osteoblasts inducing.Alkaline Phosphatase (ALP) ,type I Collagen and mineralization in vitro were applied to identify the osteoblast and evaluate its activity.Result:Human MSCs could be isolated from HUCB and bone marrow MSCs and these cells had no differences in their origin, morphology,and biological properties. The osteoblasts induced from MSCs had a positive staining for known markers:ALP and mineralization in vitro and also stable expressing of ALP and type I Collagen.Conclusion:MSCs can be induced into osteoblasts in a suitable condition,umbilical cord blood may prove to be a new source for cellular therapeutics for bone repair." @default.
- W2389474126 created "2016-06-24" @default.
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- W2389474126 date "2004-01-01" @default.
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- W2389474126 title "Osteoblasts induced from the human umbilical cord blood mesenchymal stem cells and its osteogenic potential" @default.
- W2389474126 hasPublicationYear "2004" @default.
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