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- W2389476319 abstract "Objective:To construct recombinant retrovirus vectors carrying human VSIG4 gene (VSIG4L and VSIG4S),which can be stably expressed in cell line L929,and to study the costimulatory effects to T lymphocytes and the mechanism in volved.Methods:The full length human VSIG4 genes (VSIG4L and VSIG4S) were amplified by RT-PCR from the human lung cDNA library.Digested with restriction endonuclease Pst I and EcoR I,each of the VSIG4 genes was inserted into retrovirus vector pEGZ-Term.The recombinant vector together with its two helper virus vectors was co-transfected into the package cell 293T with LipofectAMINE 2000TM.The supernatant of 293T was used to infect L929 cells.L929 cells,and stably expressing VSIG4L and VSIG4S protein was selected in the presence of zeocin,which was then analyzed by PCR and FCM.The functions of VSIG4L and VSIG4S for proliferation and IL-2 secretion of T cells were studied by MTT and ELISA respectively.Results:Both of the genes VSIG4L and VSIG4S were successfully cloned,and their recombinant retrovirus vectors were constructed.The selected L929 cells stably expressed VSIG4L and VSIG4S protein on the membrane of cells.The transfected cells have inhibitory effects on the activation,proliferation and IL-2 secretion of T cells.Conclusion:L929 cells lines stably expressing VSIG4L or VSIG4S protein were established and VSIG4 may play a negative role in regulation of T cell proliferation and IL-2 production." @default.
- W2389476319 created "2016-06-24" @default.
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- W2389476319 date "2009-01-01" @default.
- W2389476319 modified "2023-09-25" @default.
- W2389476319 title "Establishment of human VSIG4 gene transfectants and study of its costimulatory effects on T lymphocyte" @default.
- W2389476319 hasPublicationYear "2009" @default.
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