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- W2389476564 abstract "Bovine whey contains many kinds of high-abundance proteins and varied interferential impurity,so it’s hard to obtain satisfied protein images of bovine whey in two dimensional electrophoresis(2-DE).Protein extraction from bovine whey is a key step to achieve high-resolution protein separation in 2-DE.Four routine total protein extraction methods were compared in order to determine an optimal one in 2-DE analysis for bovine whey.Bovine whey protein were extracted by direct schizolysis,TCA-acetone,trizol precipitation and 2-D clean up kit.The concentration of total protein was measured by 2-D quant kit,bovine whey protein were separated using SDS-PAGE and 2-DE.The data showed that use of 2-D clean up kit gave maximum protein yield,and the use of trizol gave the lowest.The result of SDS-" @default.
- W2389476564 created "2016-06-24" @default.
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- W2389476564 date "2011-01-01" @default.
- W2389476564 modified "2023-09-25" @default.
- W2389476564 title "Comparison of Bovine Whey Protein Preparation Methods for Two-Dimensional Gel Electrophoresis Analysis" @default.
- W2389476564 hasPublicationYear "2011" @default.
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