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- W2389490012 abstract "Objective: To study the relationship between the gene expressions of ornithine decarboxylase and lung cancer in the elder from the level of mRNA and protein,and therefore to establish the theoretical basis for gene diagnosis and gene therapy in the future.Methods: RT-PCR technique was used to observe the density of ODC mRNA,and immunohistochemical method to observe the difference of ODC mRNA.Western Blot technique was used to observe the density of ODC.At the same time,MTT technique was used to observe the multiplication of differenttransfected cells.Results: The expressions of ODC mRNA and protein in lesion of lung cancer were significantly higher than those in contiguous normal tissues(P0.05),and they were quitely higher in stageⅢ than stageⅠ and stageⅡ(P0.05).The growth curve observed by MTT showed that multiplication was slower in transfected cells than in contrasts.Conclusion: ODC gene plays an important role in lung cancer and the up-regulated expression of ODC is related to the occurrence and development of lung cancer.ODC recombinant vectors could inhibit the growth of A-549 lung cancer cell lines." @default.
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- W2389490012 date "2006-01-01" @default.
- W2389490012 modified "2023-09-25" @default.
- W2389490012 title "Gene expressions of Ornithine decarboxylase in lung cancer and their clinical significances" @default.
- W2389490012 hasPublicationYear "2006" @default.
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