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- W2389543719 abstract "Objective To investigate the binding action between the severe acute respiratory syndrome coronavirus (SARS-CoV)S666 protein and the angiotensin-converting enzyme 2(ACE2)receptor in eyes.Methods The recombinant pS666-EGFP were transfected into 293T cells and expressed,the binding capability of the SARS-CoV S666 proteins with cells including vero E6 cells,conjunctival epithelial cells,conjunctival fibroblast and corneal epithelial cells and tissues including heart,lung,biain,cornea and conjunctiva were examined by enzyme-linked immunosorbent assay and flow cytometry.Results The map of restriction enzyme digestion showed the pS666-EGFP fusion gene was successfully cloned into the vector pEGFP-N1.Western Blotting showed that the relative molecular weight of fusion protein was about 100.7×103 after transfection of 293T cells with pS666-EGFP.The enzyme-linked immunosorbent assay showed,compared with the control group of EGFP proteins,the binding level of S666-EGFP proteins increased in epithelial cells,conjunctival fibroblast,corneal epithelial cells,corneal and conjunctival tissue (P0.001),but the binding capabilities of the above cells and tissues were weaker than that of vero E6 cells,heart and lung tissue.The results of flow cytometry further confirmed this results,and this interaction could be blocked by a goat anti-ACE2 antibody,which indicated that the interaction was specific.Conclusion The SARS-CoV S666 protein can bind with the conjunctival and corneal cells slightly,which is helpful not only for understanding the mechanism of SARS,but for the further study on the prevention and treatment of SARS." @default.
- W2389543719 created "2016-06-24" @default.
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- W2389543719 date "2007-01-01" @default.
- W2389543719 modified "2023-09-23" @default.
- W2389543719 title "Binding action between SARS-CoV S666 protein and ACE2 receptor in eyes" @default.
- W2389543719 hasPublicationYear "2007" @default.
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