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- W2389569560 abstract "The production of sorbitol by permeabilized cells of Zymomonas mobilis immobilized in -carrageenan was investigated. Cetyltrimethylammoniumbromide (CTAB) permeabilized cells were treated with glutaraldehyde prior to immobilization for cross-linking of enzymes, glucose-fructose oxidoreductase (GFOR) in cells. Rigidity of the immobilized beads was increased two-fold with 90 conversion efficiency by the additions of 40 (w/v) polyols (glycerol 25 g + propylene glycol 15 g) to 60 (w/v) distilled water containing 2.5 (w/v) -carrageenan as a final concentration, prior to immobilization. -Carrageenan beads entrapping permeabilized cells were dried to improve bead rigidity and storage stability. During s6mi-batch process for 72 h with dry beads, there was an improvement of the loss of enzyme activity (less than 10). In batch process, the kinetic results of value for the free cells, wet beads and dry -carrageenan beads were 71.7, 72.4 and 116.7 g/l, respectively. Higher productivity was obtained with two-stage continuous packed bed reactors with both wet and dry -carrageenan beads at 25.00 and 21.15 g/l/h, respectively, when measured at second stage." @default.
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- W2389569560 date "1996-01-01" @default.
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- W2389569560 title "Enzymatic Sorbitol Production with Zymomonas mobilis Immobilized in k-Carrageenan" @default.
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