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- W2389592076 abstract "Iron transport protein(IRT) is a unique protein that transports ion across membrane and belongs to the member of iron transport family-ZIP.The full-length IRT gene in Chorispora bungeana and its expression under different temperatures and different Fe levels were studied by using 3′ and 5′ RACE-PCR cloning and real-time quantitative PCR.Sequence alignment and phylogenetic tree analyses showed that the IRT gene in Chorispora bungeana(named CbIRT),comprising 1 290 bp with a complete open reading frame of 1 035 bp,encoding a peptide of 344 amino acid residues,was highly homologous to AtIRT1(87.4%) and TcIRT-G(86.5%),respectively,and the sequence of deduced amino acids has the 89% homology to that of AtIRT1.Expression analysis by real-time quantitative PCR revealed that CbIRT gene expression can be induced either by shorten Fe treatment or low temperature stress,while depressed by additional Fe treatment.This indicated that CbIRT gene might play key roles not only in Fe metabolism but also in low temperature stress." @default.
- W2389592076 created "2016-06-24" @default.
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- W2389592076 date "2008-01-01" @default.
- W2389592076 modified "2023-09-25" @default.
- W2389592076 title "Molecular Cloning and Analysis of IRT Gene Expression via Real-time PCR in Chorispora bungeana fisch & C.A.Mey" @default.
- W2389592076 hasPublicationYear "2008" @default.
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