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- W2389597316 abstract "Circular dichroism is an important method to study the conformation of macromolecules. Our experiment showed that Lyc distinct lyaltered CD spectra of calf thymus DNA. The degree of alteration had a close relationship with concentration of drug. At 12.5 μg/ml of Lyc, the positive peak at 274 nm of DNA spectra was of blue shift to 266 nm, and the [ψ] value increased, but the [ψ] yalue of the negative peak at 243 nmof DNA spectra enhanced, the spectrum band narrowed, and the peak pcsition was cf red shift to 245 nm. At 50 μg/ml of Lyc, the CD spectra of DNA was completely different. The negative peak was shifted to 267-289 nm and 207 nm, meanwhile the positive peak appeared at 244 nm.When the concentration was increased further, the precipitate appeared in the solution and CD spectra disappeared. After dialysis of DNA-Lyc complex. the CD spectra were basically recovered to the original form, but the peak value was lower. Lyc itself has no CD spectra. To achieve the equili brium, for low concentration of Lycthe reaction lasted 0.33 min and it needed 3.96 min for high concentration." @default.
- W2389597316 created "2016-06-24" @default.
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- W2389597316 date "1987-01-01" @default.
- W2389597316 modified "2023-09-24" @default.
- W2389597316 title "Effect of lycobetaine on DNA circular dichroism" @default.
- W2389597316 hasPublicationYear "1987" @default.
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