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- W2389607431 abstract "Objective: To compare the expression of pluripotency gene Oct4/Nanog in the differentiated human induced pluripo-tent stem cells(iPSCs) and embryonic stem cells(ESCs).Methods: The RNA of embryoid bodies(EBs) derived from undifferentiated human iPSCs and hESCs at different time of differentiation were collected.The gene expression related to three-layer differentiation and pluripotency gene Oct4/Nanog was detected by RT-PCR.The Oct4 positive cells in the teratoma derived from both human iPSCs and hESCs were detected by immunofluorescence staining.Results: The expression of endoderm and ectroderm related gene in the EBs derived from iPSCs was later than that derived from hESCs.The expression of Oct4 and Nanog was still existence in D18 EBs derived from undifferentiated human iPSCs.The Oct4 positive cells were detected in iPSCs derivded-teratoma.Conclusion: Although the iPSCs can differentiate into three-layer cells,the higher expression of pluriotency gene Oct4/Nanog was maintained in the differentiated iPSCs generated by lentivirus infection than hES." @default.
- W2389607431 created "2016-06-24" @default.
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- W2389607431 date "2011-01-01" @default.
- W2389607431 modified "2023-09-24" @default.
- W2389607431 title "Comparison of Human Induced Pluripotent Stem Cells and Oct4/Nanog Gene Expression in Human Embryonic Stem Cell Differentiation" @default.
- W2389607431 hasPublicationYear "2011" @default.
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