Matches in SemOpenAlex for { <https://semopenalex.org/work/W2389610012> ?p ?o ?g. }
Showing items 1 to 55 of
55
with 100 items per page.
- W2389610012 abstract "This paper deals with the molecular mechanism of the infection of Penaeus chinensis by white spot syndrome virus. A stain of WSSV was isolated and purified from P. chinensis with sucrose gradient centrifugation. Protein VP28 was identified by SDS-PAGE. Genome DNA of WSSV was extracted. The primer was designed and synthesized according to the reported sequence in the GenBank. The gene of VP28 protein was cloned from Genome DNA with PCR amplification. The product of PCR digested by restriction enzyme BamHI and EcoRI was constructed on the clone vector pUC19. The recombinant vector was identified with double digestion of BamHI and EcoRI. The gene was sequenced and the results showed that the VP28 gene contains 615 nucleotides encoding 204 amino acids and 100% identity with WSSV sequences reported in GenBank. The clone vector was named as pUC-VP28. Then, plasmid pUC-VP28 digested by BamHI (3.3 kb) was ligased with pRL-489 digested by BamHI (9.0 kb). Recombinant was selected with double antibiotics and was identified with digestion with BamHI. The VP28 gene inserted at the same site at downstream of the promoter PpsbA moves towards different directions. There are two EcoRI sites: one is at upstream of the promoter PpsbA and the other located at the 3' end of vp28 gene. The recombinant was digested with EcoRI and 1.0kb was seen in the gel of 0.6%. The result showed that the vp28 gene was located at the downstream of PpsbA. The recombinant shuttle expression vector for cyanobacteria was named as pRL-VP28. It was the key step for expression of VP28 protein in cyanobacteria." @default.
- W2389610012 created "2016-06-24" @default.
- W2389610012 creator A5086038672 @default.
- W2389610012 date "2003-01-01" @default.
- W2389610012 modified "2023-09-26" @default.
- W2389610012 title "CLONE OF ENVELOPE PROTEIN VP28 GENE OF WHITE SPOT SYNDROME VIRUS (WSSV) AND EXPRESSION VECTOR CONSTRUCTION FOR CYANOBACTERIA" @default.
- W2389610012 hasPublicationYear "2003" @default.
- W2389610012 type Work @default.
- W2389610012 sameAs 2389610012 @default.
- W2389610012 citedByCount "0" @default.
- W2389610012 crossrefType "journal-article" @default.
- W2389610012 hasAuthorship W2389610012A5086038672 @default.
- W2389610012 hasConcept C104317684 @default.
- W2389610012 hasConcept C128319531 @default.
- W2389610012 hasConcept C130123407 @default.
- W2389610012 hasConcept C153911025 @default.
- W2389610012 hasConcept C40767141 @default.
- W2389610012 hasConcept C42174275 @default.
- W2389610012 hasConcept C54355233 @default.
- W2389610012 hasConcept C86803240 @default.
- W2389610012 hasConceptScore W2389610012C104317684 @default.
- W2389610012 hasConceptScore W2389610012C128319531 @default.
- W2389610012 hasConceptScore W2389610012C130123407 @default.
- W2389610012 hasConceptScore W2389610012C153911025 @default.
- W2389610012 hasConceptScore W2389610012C40767141 @default.
- W2389610012 hasConceptScore W2389610012C42174275 @default.
- W2389610012 hasConceptScore W2389610012C54355233 @default.
- W2389610012 hasConceptScore W2389610012C86803240 @default.
- W2389610012 hasLocation W23896100121 @default.
- W2389610012 hasOpenAccess W2389610012 @default.
- W2389610012 hasPrimaryLocation W23896100121 @default.
- W2389610012 hasRelatedWork W2337119821 @default.
- W2389610012 hasRelatedWork W2362940824 @default.
- W2389610012 hasRelatedWork W2367252687 @default.
- W2389610012 hasRelatedWork W2368259936 @default.
- W2389610012 hasRelatedWork W2368759507 @default.
- W2389610012 hasRelatedWork W2369140318 @default.
- W2389610012 hasRelatedWork W2373999656 @default.
- W2389610012 hasRelatedWork W2374984843 @default.
- W2389610012 hasRelatedWork W2377036045 @default.
- W2389610012 hasRelatedWork W2379396970 @default.
- W2389610012 hasRelatedWork W2388281399 @default.
- W2389610012 hasRelatedWork W2388677117 @default.
- W2389610012 hasRelatedWork W2391213523 @default.
- W2389610012 hasRelatedWork W2473763524 @default.
- W2389610012 hasRelatedWork W2474160156 @default.
- W2389610012 hasRelatedWork W2969344339 @default.
- W2389610012 hasRelatedWork W2970762238 @default.
- W2389610012 hasRelatedWork W3020966208 @default.
- W2389610012 hasRelatedWork W32335561 @default.
- W2389610012 hasRelatedWork W2550721488 @default.
- W2389610012 isParatext "false" @default.
- W2389610012 isRetracted "false" @default.
- W2389610012 magId "2389610012" @default.
- W2389610012 workType "article" @default.