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- W2389621027 abstract "OBJECTIVE To study the isolation and anti-tumor activity of exopolysaccharide(REPS) from Rhizobium sp.N613.METHODS The fermentation broth was pretreated by acidification,heating,adding coagulant and flocculant.The ethanol precipitation conditions of REPS was optimized by an orthogonal design.REPS was deproteined by Sevag method,and homogeneity was determined by gel column chromatography and UV spectrum.Transplanted tumor models of sarcoma 180 in mice were used to evaluate the anti-tumor effect.The white blood cell and lymphocyte of blood samples from S180 bearing mice were counted.RESULTS The process of fermentation broth pretreatment adjusted pH value to 5.0 with HCl at 70 ℃ for 20 min,with the addition of 5 g·L-1 Al2(SO4)3·18H2O and 0.2 g·L-1 sodium alginate.The optimized conditions of REPS ethanol precipitation were as follows:pH value 7.0,65% ethanol and precipitation time for 8 h.REPS was homogeneous polysaccharide.The result of anti-tumor activity showed that inhibition rate was 44.17%.Compared with the S180 bearing group,REPS significantly promoted the proliferation of white blood cell and lymphocyte.CONCLUSION The parameters of fermentation broth pretreatment and the conditions of REPS ethanol precipitation were obtained.REPS inhibited significantly the growth of S180." @default.
- W2389621027 created "2016-06-24" @default.
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- W2389621027 date "2008-01-01" @default.
- W2389621027 modified "2023-09-27" @default.
- W2389621027 title "Studies on Isolation and Anti-S180 Activity of Exopolysaccharide from Rhizobium sp.N613" @default.
- W2389621027 hasPublicationYear "2008" @default.
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