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- W2389632906 abstract "To provide basis for introducing LMV-CP into the Chinese cabbage genome and its high effective and permanent expression,experiments were conducted under the optimum conditions including soaking the outplant by Agrobacterium for 15 min,putting AS into the media during the process of Agrobacterium cultivation and co-cultivation,keeping the pH of media at 5.2.After precultivation for 3 days, the cotyledon petioles were co-cultivated for 3 days with Agrobacterium C58 harhouring binary vectors carrying Bar gene for Basta resistance and LMV-CP gene, then the cotyledon petioles were placed in the selective medium containing 5 mg/L Basta. Shoot induction continued for 4～6 weeks. The shoots were transferred to the root induction medium. Resistant plants frequency was 6%. Through polymerase chain reaction (PCR) analyses and PCR-Southern dot blot hybridization analyses, 3 of 6 resistant plants, which were randomly selected, confirmed the introduction of the LMV-CP into the Chinese cabbage genome." @default.
- W2389632906 created "2016-06-24" @default.
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- W2389632906 date "2001-01-01" @default.
- W2389632906 modified "2023-09-22" @default.
- W2389632906 title "Transformation of Chinese Cabbage (B.campestris L.ssp.Pekinensis) by Using Agrobacterium" @default.
- W2389632906 hasPublicationYear "2001" @default.
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