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- W2389654708 abstract "A dicotyledon expression vector was constructed for transforming pinosylvinsynthase gene from Pinus massoniana.This vector contains 35S promoter and NOS terminator,which can drive the gene's high expression in dicotyledon.Primers with BglⅡand BstEⅡwere designed and PCR was used to amplify the pinosylvin synthase gene.A new cloning vector was then constructed by inserting the amplified pinosylvin synthase gene into pCAMBIA1301 expression vector.PCR and restriction analysis showed that the inserted DNA fragment was the size of expecting.Furthermore,sequencing showed that the inserted DNA fragment was not out of frame.The expression vector was then transferred into Agrobacterium tumefaclens LBA4404,which was the foundation to function research of pinosylvin synthase." @default.
- W2389654708 created "2016-06-24" @default.
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- W2389654708 date "2008-01-01" @default.
- W2389654708 modified "2023-09-24" @default.
- W2389654708 title "Construction and Characterization of Dicotyledon Expression Vector Containing Pinosylvin Synthase Gene from Pinus massoniana" @default.
- W2389654708 hasPublicationYear "2008" @default.
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