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- W2389662906 abstract "Objective:To highly expression of Pfu in E.coli.Method:pET28a-pfu was successfully constructed by inserting pfu gene into pET28a,the recombinant DH5α was obtained by transforming pET28a-pfu into E.coli DH5α.When OD600 of recombinant DH5α was 0.2,pfu was inducibly expressed by using IPTG at the final concentration of 1 mmol/L.10-12 h later,recombinant DH5α pellet was heated at 80 ℃ for 30 min after wall-broken by ultrasonic,The extraction was furified by metal-affinity chromatography on Ni2+-Sepharose columns.Result:High-quality-purified Pfu was obtained,The pfu DNA polymerase activity was confirmed by successf Lly amplification of a target DNA through PCR.Conclusion:It was confirmed that the Pfu enzyme was highly expressed and characterized by high DNA polymerase activity." @default.
- W2389662906 created "2016-06-24" @default.
- W2389662906 creator A5003281346 @default.
- W2389662906 date "2012-01-01" @default.
- W2389662906 modified "2023-09-26" @default.
- W2389662906 title "Highly-Expression and Activity Analysis of pfu DNA Polymerase in E.coli" @default.
- W2389662906 hasPublicationYear "2012" @default.
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