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- W2389668891 abstract "[Objective]To study the alterations of biological behavior and the expressions of HPV16/E6E7 and ERβ of immortalized cervical cell line End1/E6E7 affected by 17-β-E 2, and to investigate the action to estrogen and its receptor with HPV in the progression of cervical lesions. [Methods] The cytometrial curve, MTT assay and flow cytometry assay(FCM) were used to study the growth and proliferation of the cell line regulated by 17-β-estrodial when ERα was negative and ERβ was positive, and the expression of HPV16/E6E7 and ERβ were evaluated by reverse transcription-polymerase chain reaction(RT-PCR) simultaneously. [Results] The proliferation of the End1/E6E7 cell line and the expression of HPV16/E6E7 were promoted by the effect of E 2 with the concentration varying from 0.01×10 -6 M to 0.5×10 -6 M. The expression of ERβ was not altered significantly. [Conclusions] E 2 maybe activate the growth and proliferation of immortalize cervical epithelium by promoting the expression of HPV16/E6E7 with non-ERβ mechanisms." @default.
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- W2389668891 date "2004-01-01" @default.
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- W2389668891 title "Study of estrodial inducing immortalized cervical cell line Endl/E6E7 to proliferate" @default.
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