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- W2389683774 abstract "Objective : To obtain information on the growth inhibition effect of arsenic compounds and gene expression profiles using cDNA microarray technique in SiHa cell lines. Methods : We cultured SiHa cell in 96 well plate and we investigated growth inhibition effects using MTT assay and also we performed gene expression profile experiment using 384 cDNA chip in SiHa cell after exposure of arsenics (, - 1 M) for 48 hrs. Results : Arsenics(, ) inhibit the growth of SiHa cells (: 0.5, 1, 2, 3, 4, 5 M - 9.2, 56, 89, 93, 96, 96%, : 0.5, 1, 2, 3, 4, 5 M- 54, 84, 84, 85, 85, 87%) in 4 days culture. and induced apoptosis in SiHa cells. After exposure of, 47 genes were changed more than 2 times (eg, thymidylate synthetase, cyclin B1, CDC 20). In case of , 78 genes were changed more than 2 times (eg, CDC 20, cyclin B1, primase, proliferating cell nuclear antigen). Conclusion : we observed arsenic compound (, ) inhibit the growth of SiHa cell. In gene expression profiling experiment, 78 genes was changed the expression level 2 times more than that of reference RNA after treatment of and 47 genes after treatment of . Through these result, we thought more study need in functional genomics after arsenic treated cervical cancer cells." @default.
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- W2389683774 date "2002-07-01" @default.
- W2389683774 modified "2023-09-27" @default.
- W2389683774 title "The Gene Expression Profile Using cDNA microarray after treatment Arsenic Compound (As2O3, As4O6) in SiHa Cell." @default.
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