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- W2389726500 abstract "The expression of the immunogenic glycoprotein D(gD) of pseudorabies virus (PRV) in transgenic tobacco was reported. The modified binary vector pBI 35SL was constructed by replacing the single cauliflower mosaic virus (CaMV) 35 S and GUS gene of pBI121 with modified CaMV 35 S promoter from pRTL NS2. The resulting vector had a CaMV 35 S promoter with duplicated enhancer region fused to the tobacco etch virus (TEV) 5′ untranslated region (UTR). Plant expression vector pBI gD was made by sub cloning the 1 2 kb fragment coding the full length gD of PRV under the control of the modified CaMV 35 S promoter. Tobacco was transformed by co cultivating leaf discs with %Agrobacterium% strains harboring pBI gD . The regenerated transformants were analyzed by PCR and Southern hybridization. The results indicated that the gD gene was integrated into the genomic DNA of the transgenic tobacco. Dot ELISA and Western blotting further demonstrated the expression and antigenicity of gD in transgenic tobacco." @default.
- W2389726500 created "2016-06-24" @default.
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- W2389726500 date "2002-01-01" @default.
- W2389726500 modified "2023-09-27" @default.
- W2389726500 title "Expression of the gD Gene of Pseudorabies Virus in Transgenic Tobacco" @default.
- W2389726500 hasPublicationYear "2002" @default.
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