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- W2389770063 abstract "Objective To investigate the influence of PTEN protein expression on the invasive and adhesive ability of colorectal cancer cell. Methods 1. Protein expression of PTEN were tested in three colorectal cancer cell line with different metastasic ability by western-blot.2.The human PTEN cDNA containing eukaryotic expression vectors pcDNA3.0 was transferred into human colorectal cancer cell line LOVO, to investigate the changes of invasive and adhesive ability of LOVO by Boyden chamber and a microscope slide combined with Laminin. Results 1. The content of PTEN protein in LOVO, which had a higher ability of mestastase lower than that in HT-29,LS-174T. 2.The adhesive rate of LOVO and LOVO/pcDNA3.0-PTEN to laminin at 20mins is 18.6% ±1.4% and 13.9% ±0.48%(P0.05),at 120mins is 71.2% ±2.5% and 56.0% ±1.6% (P0.05)respectively,3. The average number of LOVO and LOVO/pcDNA3.0-PTEN,whitch could transit through Boyden chamber was 11.7±1.74 and 7.5 ±1.58个(P0.05), Conclusion The expression of PTEN may play an important role in the metastase process of colorectal cancer." @default.
- W2389770063 created "2016-06-24" @default.
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- W2389770063 date "2003-01-01" @default.
- W2389770063 modified "2023-09-25" @default.
- W2389770063 title "The influence of PTEN protein expression on the invasive and sdhesive ability of colorectal cancer cell" @default.
- W2389770063 hasPublicationYear "2003" @default.
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