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- W2389774313 abstract "Objective To construct the stable Smad7 transfecting glioblastoma cell lines and investigate the effect of Smad7 on the growth of glioblastoma cells.Methods The eukaryotic expression plasmid pCDNA3.0-Smad7 and empty vector pCDNA3.0 were transfected into U251 cells with lipofectine,G418 was used for selecting the positive clone,and the effects of Smad7 on cell proliferation was measured by cell growth curve.Results Not only at the level of transcription but also translation,Smad7 expressed much higher in Smad7 transfectant than that in U251 cells and blank pCDNA3.0 vector transfection confirmed by RT-PCR and western blot respectively.Moreover,the cells grow slower after Smad7 transfection.Conclusion The stable smad7 transfect glioblastoma cell line was constructed successfully,and exogenous Smad7 gene overexpression could inhibit cell proliferation." @default.
- W2389774313 created "2016-06-24" @default.
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- W2389774313 date "2009-01-01" @default.
- W2389774313 modified "2023-09-26" @default.
- W2389774313 title "Construction of the stable Smad7 transfecting glioblastoma cell lines" @default.
- W2389774313 hasPublicationYear "2009" @default.
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