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- W2389781331 abstract "AIM: To investigate the main characteristics of cardiac Ca 2+ sparks evoked by loose-patch method. METHODS: Loose-seal patch-clamp combined with confocal microscopic Ca 2+ imaging (Loose-patch Method) was used. RESULTS: The probability for the Loose-patch Method to evoke in-focus Ca 2+ sparks was 72.2%, which was much higher than the activation of spontaneous Ca 2+ sparks. The amplitude for the averaged Ca 2+ sparks evoked by Loose-patch Method was (1.07±0.06) (in ΔF/F 0, the normalized fluorescence, where F 0 denotes the resting fluorescence) and the averaged spatial width was (1.44±0.03) μm ( n =124). CONCLUSION: Loose-patch Method, while maintaining the normal signaling coupling between L-type Ca 2+ channel and ryanodine receptors, can evoke Ca 2+ sparks and the characteristics of Ca 2+ sparks by activating Ca 2+ release locally and in-focus can be recorded by confocal microscope." @default.
- W2389781331 created "2016-06-24" @default.
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- W2389781331 date "2004-01-01" @default.
- W2389781331 modified "2023-09-23" @default.
- W2389781331 title "Ca~(2+) sparks evoked by loose-patch method" @default.
- W2389781331 hasPublicationYear "2004" @default.
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